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影响因子：17.521

DOI码：10.1002/advs.202105588

发表刊物：Advanced Science

关键字：GCaMP6f brain activation brain imaging electric paw stimulation epifluorescence functional optoacoustic tomography hemodynamic response neuroimaging

摘要：Modern optical neuroimaging approaches are expanding the ability to elucidate complex brain function. Diverse imaging contrasts enable direct observation of neural activity with functional sensors along with the induced hemodynamic responses. To date, decoupling the complex interplay of neurovascular coupling and dynamical physiological states has remained challenging when employing single-modality functional neuroimaging readings. A hybrid fluorescence optoacoustic tomography platform combined with a custom data processing pipeline based on statistical parametric mapping is devised, attaining the first noninvasive observation of simultaneous calcium and hemodynamic activation patterns using optical contrasts. Correlated changes in the oxy- and deoxygenated hemoglobin, total hemoglobin, oxygen saturation, and rapid GCaMP6f fluorescence signals are observed in response to peripheral sensory stimulation. While the concurrent epifluorescence serves to corroborate and complement the functional optoacoustic observations, the latter further aids in decoupling the rapid calcium responses from the slowly varying background in the fluorescence recordings mediated by hemodynamic changes. The hybrid imaging platform expands the capabilities of conventional neuroimaging methods to provide more comprehensive functional readings for studying neurovascular and neurometabolic coupling mechanisms and related diseases.

合写作者：Zhou, Quanyu,Reiss, Michael,Deán Ben, Xosé Luís,Gezginer, Irmak,Ni, Ruiqing

第一作者：Chen, Zhenyue

论文类型：期刊论文

通讯作者：Shoham, Shy,Razansky, Daniel

卷号：9

期号：24

页面范围：e2105588

是否译文：否

发表时间：2022-08-01

收录刊物：SCI