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影响因子：29.234

DOI码：10.1038/s41551-022-00906-1

发表刊物：Nature Biomedical Engineering

摘要：Deposits of amyloid-beta (Abeta) in the brains of rodents can be analysed by invasive intravital microscopy on a submillimetre scale, or via whole-brain images from modalities lacking the resolution or molecular specificity to accurately characterize Abeta pathologies. Here we show that large-field multifocal illumination fluorescence microscopy and panoramic volumetric multispectral optoacoustic tomography can be combined to longitudinally assess Abeta deposits in transgenic mouse models of Alzheimer's disease. We used fluorescent Abeta-targeted probes (the luminescent conjugated oligothiophene HS-169 and the oxazine-derivative AOI987) to transcranially detect Abeta deposits in the cortex of APP/PS1 and arcAbeta mice with single-plaque resolution (8 mum) and across the whole brain (including the hippocampus and the thalamus, which are inaccessible by conventional intravital microscopy) at sub-150 mum resolutions. Two-photon microscopy, light-sheet microscopy and immunohistochemistry of brain-tissue sections confirmed the specificity and regional distributions of the deposits. High-resolution multiscale optical and optoacoustic imaging of Abeta deposits across the entire brain in rodents thus facilitates the in vivo study of Abeta accumulation by brain region and by animal age and strain.

合写作者：Voigt, Fabian F,Zhou, Quanyu,Villois, Alessia,Shi, Gloria,Kirschenbaum, Daniel,Helmchen, Fritjof

第一作者：Ni, Ruiqing,Chen, Zhenyue,Deán-Ben, Xosé Luís

通讯作者：Klohs, Jan,Razansky, Daniel

卷号：6

期号：9

页面范围：1031-1044

ISSN号：2157-846X

是否译文：否

发表时间：2022-07-14

收录刊物：SCI