Multiscale optical and optoacoustic imaging of amyloid-β deposits in mice
Impact Factor:29.234
DOI number:10.1038/s41551-022-00906-1
Journal:Nature Biomedical Engineering
Abstract:Deposits of amyloid-beta (Abeta) in the brains of rodents can be analysed by invasive intravital microscopy on a submillimetre scale, or via whole-brain images from modalities lacking the resolution or molecular specificity to accurately characterize Abeta pathologies. Here we show that large-field multifocal illumination fluorescence microscopy and panoramic volumetric multispectral optoacoustic tomography can be combined to longitudinally assess Abeta deposits in transgenic mouse models of Alzheimer's disease. We used fluorescent Abeta-targeted probes (the luminescent conjugated oligothiophene HS-169 and the oxazine-derivative AOI987) to transcranially detect Abeta deposits in the cortex of APP/PS1 and arcAbeta mice with single-plaque resolution (8 mum) and across the whole brain (including the hippocampus and the thalamus, which are inaccessible by conventional intravital microscopy) at sub-150 mum resolutions. Two-photon microscopy, light-sheet microscopy and immunohistochemistry of brain-tissue sections confirmed the specificity and regional distributions of the deposits. High-resolution multiscale optical and optoacoustic imaging of Abeta deposits across the entire brain in rodents thus facilitates the in vivo study of Abeta accumulation by brain region and by animal age and strain.
Co-author:Voigt, Fabian F,Zhou, Quanyu,Villois, Alessia,Shi, Gloria,Kirschenbaum, Daniel,Helmchen, Fritjof
First Author:Ni, Ruiqing,Chen, Zhenyue,Deán-Ben, Xosé Luís
Correspondence Author:Klohs, Jan,Razansky, Daniel
Volume:6
Issue:9
Page Number:1031-1044
ISSN No.:2157-846X
Translation or Not:no
Date of Publication:2022-07-14
Included Journals:SCI